{"id":31,"date":"2020-03-02T14:32:08","date_gmt":"2020-03-02T13:32:08","guid":{"rendered":"https:\/\/wp.modronebo.net\/ijs2\/?page_id=31"},"modified":"2020-05-15T09:22:50","modified_gmt":"2020-05-15T08:22:50","slug":"glycobiology","status":"publish","type":"page","link":"https:\/\/www-b3.ijs.si\/index.php\/research\/research-fields\/glycobiology\/","title":{"rendered":"Microbial glycobiology"},"content":{"rendered":"<p><section class=\"kc-elm kc-css-436551 kc_row\"><div class=\"kc-row-container  kc-container\"><div class=\"kc-wrap-columns\"><div class=\"kc-elm kc-css-596560 kc_col-sm-12 kc_column kc_col-sm-12\"><div class=\"kc-col-container\"><div class=\"kc-elm kc-css-60013 kc_text_block\"><\/p>\n<h2><!-- &#091;if gte mso 9&#093;><xml><br \/><o:OfficeDocumentSettings><br \/><o:AllowPNG><\/o:AllowPNG><br \/><\/o:OfficeDocumentSettings><br \/><\/xml><!&#091;endif&#093;--><!-- &#091;if gte mso 9&#093;><xml><br \/><w:WordDocument><br \/><w:View>Normal<\/w:View><br 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Normal\";<br \/>\tmso-tstyle-rowband-size:0;<br \/>\tmso-tstyle-colband-size:0;<br \/>\tmso-style-noshow:yes;<br \/>\tmso-style-priority:99;<br \/>\tmso-style-parent:\"\";<br \/>\tmso-padding-alt:0cm 5.4pt 0cm 5.4pt;<br \/>\tmso-para-margin-top:0cm;<br \/>\tmso-para-margin-right:0cm;<br \/>\tmso-para-margin-bottom:8.0pt;<br \/>\tmso-para-margin-left:0cm;<br \/>\tline-height:107%;<br \/>\tmso-pagination:widow-orphan;<br \/>\tfont-size:11.0pt;<br \/>\tfont-family:\"Calibri\",\"sans-serif\";<br \/>\tmso-ascii-font-family:Calibri;<br \/>\tmso-ascii-theme-font:minor-latin;<br \/>\tmso-hansi-font-family:Calibri;<br \/>\tmso-hansi-theme-font:minor-latin;<br \/>\tmso-bidi-font-family:\"Times New Roman\";<br \/>\tmso-bidi-theme-font:minor-bidi;<br \/>\tmso-ansi-language:EN-GB;}<br \/><\/style>\n\n\n\n\n\n\n\n\n<p><!&#091;endif&#093;--><\/h2>\n<h2>Glycobiology and fungal proteins<\/h2>\n<p>Glycobiology studies structure, synthesis, role and evolution of carbohydrates in biological systems.\u00a0 Carbohydrates are important molecules of life that provide incredible complexity to interactions between cells in an organism and between organisms. The group of proteins that mediate molecular interactions through carbohydrates are called lectins.\u00a0 Higher fungi have been shown to be a rich source of proteins with unique features that distinguish them from similar proteins from other sources. We have identified and characterized several classes of proteins with focus on novel protease inhibitors and lectins from different higher fungi species. We use these interesting proteins as tools to study: <\/p>\n<ul>\n<li>molecular interactions involved in bacterial adhesion and biofilm formation, <\/li>\n<li>molecular interactions of these proteins with cancer cells and cells of the immune system,<\/li>\n<li>how these proteins affect growth and development of major invasive insect pests.<\/li>\n<\/ul>\n<p>Our goal is to investigate their potential to modify crucial molecular interactions and apply our findings to target applications in human and veterinary medicine, agriculture, bioprocessing and pharmaceutical industry.<br \/>We believe that glycans are at the forefront of molecular interactions and that using fungal proteins will help us better understand the underlying molecular mechanisms of actions, which can offer new insights for development of new strategies to: <\/p>\n<ul>\n<li>combat the occurrence of antibiotic resistance (AMR), <\/li>\n<li>develop cancer immunotherapy, and <\/li>\n<li>develop biopesticides against an increasing number of invasive alien crop pests.\n<\/li>\n<\/ul>\n<p>\n<\/div><div class=\"kc-elm kc-css-931384\" style=\"height: 30px; clear: both; width:100%;\"><\/div><\/div><\/div><\/div><\/div><\/section><section class=\"kc-elm kc-css-584783 kc_row\"><div class=\"kc-row-container  kc-container\"><div class=\"kc-wrap-columns\"><div class=\"kc-elm kc-css-157292 kc_col-sm-12 kc_column kc_col-sm-12\"><div class=\"kc-col-container\"><div class=\"kc-elm kc-css-849642 kc_shortcode kc_single_image\">\n\n        <img src=\"https:\/\/www-b3.ijs.si\/wp-content\/uploads\/2020\/08\/shema-glcofun-final-eng_2560px.jpg\" class=\"\" alt=\"\" \/>    <\/div>\n<div class=\"kc-elm kc-css-910280\" style=\"height: 30px; clear: both; width:100%;\"><\/div><\/div><\/div><\/div><\/div><\/section><section class=\"kc-elm kc-css-73321 kc_row\"><div class=\"kc-row-container  kc-container\"><div class=\"kc-wrap-columns\"><div class=\"kc-elm kc-css-322904 kc_col-sm-12 kc_column kc_col-sm-12\"><div class=\"kc-col-container\"><div class=\"kc-elm kc-css-289333 kc_text_block\"><\/p>\n<h4>Collaborations<\/h4>\n<p>Prof. Dr. Markus K\u00fcnzler, Institute of Microbiology, ETH Z\u00fcrich<br \/>Dr. Annabelle Varrot, CERMAV-CNRS Grenoble, France<br \/>Dr. Mario Schubert, Institute of Molecular Biology, University of Salzburg, Austria<br \/>Prof. Paola Cescutti, Department of Life Sciences, University of Trieste, Italy<br \/>Prof. Claire Veneault-Fourrey, Universit\u00e9 de Lorraine, INRA, France<br \/>Dr Jonathan Plett, Hawkesbury Institute for the Environment, Western Sydney University, Sydney, Australia<br \/>Dr Paul J. Hensbergen, Center for Proteomics and Metabolomics, Leiden University Medical Center, Leiden, The Netherlands<br \/>Dr. Inga Matijo\u0161yt\u0117, Vilnius University Life Science Center, Vilnius, Lithuania<br \/>Prof. Iain Wilson, Department of Chemistry, University of Natural Resources and Life Sciences, Vienna, Austria<br \/>Dr Stefan Toepfer, CABI, c\/o Plant Protection and Soil Conservation Directorate, Hodmezovasarhely, Hungary<br \/>Dr. Anja Klan\u010dnik, Department of Food Science and Technology, Biotechnical Faculty, University of Ljubljana, Slovenia<br \/>Dr. Du\u0161an Turk, Department of Biochemistry and Molecular and Structural Biology, Jo\u017eef Stefan Institute, Ljubljana, Slovenia<br \/>Dr Jaka Razinger, Plant Protection Department, Agricultural Institute of Slovenia, Ljubljana, Slovenia<br \/>Dr Janez Pre\u0161ern, Animal Production Department, Agricultural Institute of Slovenia, Ljubljana, Slovenia<br \/>Dr Nada \u017dnidar\u0161i\u010d, Department of Biology, Biotechnical Faculty, University of Ljubljana, Slovenia<br \/>Dr Ana Rotter, Marine Biology Station, National Institute of Biology, Piran, Slovenia<\/p>\n<\/p>\n<p>\n<\/div><\/div><\/div><\/div><\/div><\/section><section class=\"kc-elm kc-css-803587 kc_row\"><div class=\"kc-row-container  kc-container\"><div class=\"kc-wrap-columns\"><div class=\"kc-elm kc-css-666111 kc_col-sm-12 kc_column kc_col-sm-12\"><div class=\"kc-col-container\"><div class=\"kc-elm kc-css-317742 kc_text_block\"><\/p>\n<h4>Selected publications<\/h4>\n<ul>\n<li>STERNI\u0160A, Meta, SABOTI\u010c, Jerica, KLAN\u010cNIK, Anja. <strong><a href=\"https:\/\/www.sciencedirect.com\/science\/article\/pii\/S0168160521004803\" target=\"_blank\" rel=\"noopener\">A novel approach using growth curve analysis to distinguish between antimicrobial and anti-biofilm activities against <em>Salmonella<\/em>.<\/a><\/strong> International journal of food microbiology. 2021, 1-33.<\/li>\n<li>SABOTI\u010c, Jerica, OHM, Robin, K\u00dcNZLER, Markus. <a href=\"https:\/\/link.springer.com\/article\/10.1007\/s00253-015-7075-2\" target=\"_blank\" rel=\"noopener noreferrer\"><strong>Entomotoxic and nematotoxic lectins and protease inhibitors from fungal fruiting bodies.<\/strong><\/a> Applied microbiology and biotechnology, 2016, 100, 1, 91\u2010111.<\/li>\n<li>JANE\u017d, Nikolaja, LAD\u00c1NYI, M\u00e1rta, STERNI\u0160A, Meta, JUG, Bla\u017e, ZUPAN, Tanja, PETERNEL, Tja\u0161a, SEBASTIJANOVI\u0106, Aleksandar, PERI\u0160I\u0106, Milica, KARNI\u010cAR, Katarina, TALER-VER\u010cI\u010c, Ajda, TURK, Du\u0161an, KLAN\u010cNIK, Anja, \u0160TRANCAR, Janez, SABOTI\u010c, Jerica. <strong><a href=\"https:\/\/enviromicro-journals.onlinelibrary.wiley.com\/doi\/10.1111\/1751-7915.70040\" target=\"_blank\" rel=\"noopener\">Exposure to specific fungal lectins during adhesion impairs biofilm formation of Listeria on polystyrene<\/a>.<\/strong> Microbial biotechnology. 2024, 17, 12: e70040<\/li>\n<li><u>JANE\u017d, Nikolaja<\/u>, \u0160KRLJ, Bla\u017e, STERNI\u0160A, Meta, KLAN\u010cNIK, Anja, <u>SABOTI\u010c, Jerica<\/u>. <a href=\"https:\/\/sfamjournals.onlinelibrary.wiley.com\/doi\/full\/10.1111\/1751-7915.13847\"><strong>The role of the <em>Listeria monocytogenes<\/em> surfactome in biofilm formation<\/strong><\/a>. <em>Microbial biotechnology<\/em>, 2021, 14, 4, 1269-1281.<\/li>\n<li>SABOTI\u010c, Jerica, BAYRAM, Engin, JANE\u017d, Nikolaja, VASQUEZ, Marlen I., et al. <a href=\"https:\/\/www.sciencedirect.com\/science\/article\/pii\/S0734975024000016?via%3Dihub\" target=\"_blank\" rel=\"noopener\"><strong>A guide to the use of bioassays in exploration of natural resources<\/strong><\/a><strong>.<\/strong> Biotechnology advances. 2024, 71: 108307, str. 1-36<\/li>\n<\/ul>\n<p>\n<\/div><div class=\"kc-elm kc-css-322535\" style=\"height: 30px; clear: both; width:100%;\"><\/div><\/div><\/div><\/div><\/div><\/section><section class=\"kc-elm kc-css-674545 kc_row\"><div class=\"kc-row-container  kc-container\"><div class=\"kc-wrap-columns\"><div class=\"kc-elm kc-css-292453 kc_col-sm-12 kc_column kc_col-sm-12\"><div class=\"kc-col-container\"><div class=\"kc-elm kc-css-514533\" style=\"height: 30px; clear: both; width:100%;\"><\/div><\/div><\/div><\/div><\/div><\/section><\/p>\n","protected":false},"excerpt":{"rendered":"","protected":false},"author":6,"featured_media":681,"parent":215,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":[],"_links":{"self":[{"href":"https:\/\/www-b3.ijs.si\/index.php\/wp-json\/wp\/v2\/pages\/31"}],"collection":[{"href":"https:\/\/www-b3.ijs.si\/index.php\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www-b3.ijs.si\/index.php\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www-b3.ijs.si\/index.php\/wp-json\/wp\/v2\/users\/6"}],"replies":[{"embeddable":true,"href":"https:\/\/www-b3.ijs.si\/index.php\/wp-json\/wp\/v2\/comments?post=31"}],"version-history":[{"count":1,"href":"https:\/\/www-b3.ijs.si\/index.php\/wp-json\/wp\/v2\/pages\/31\/revisions"}],"predecessor-version":[{"id":1710,"href":"https:\/\/www-b3.ijs.si\/index.php\/wp-json\/wp\/v2\/pages\/31\/revisions\/1710"}],"up":[{"embeddable":true,"href":"https:\/\/www-b3.ijs.si\/index.php\/wp-json\/wp\/v2\/pages\/215"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www-b3.ijs.si\/index.php\/wp-json\/wp\/v2\/media\/681"}],"wp:attachment":[{"href":"https:\/\/www-b3.ijs.si\/index.php\/wp-json\/wp\/v2\/media?parent=31"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}